The main Nile Basin Development Challenge partners, working together with the International Water Management Institute (IWMI) and the International Livestock Research Institute (ILRI) - acting as co-leaders of this Challenge - have been:
- Abay Basin Authority
- Amhara Regional Agricultural Research Institute (ARARI), Bahir Dar, Ethiopia
- Catholic Relief Services- Ethiopia (CRS), Addis Ababa, Ethiopia
- Deutsche Gesellschaft for Internationale Zusammenarbeit (GIZ/SLM)
- Ethiopian Economic Association (EEA), Addis Ababa, Ethiopia
- Ethiopian Evagelical Church Mekane Eyesus Development and Social Services Commission (EECMY-DASSC)
- Ethiopian Institute of Agricultural Research (EIAR)
- Ethio-Wet Land and Natural Resource Association
- HUNDEE-Grass Root Development Initiative
- Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture (IRSTEA), Montpellier, France
- Ministry of Agricuylture (MoA), Addis Ababa, Ethiopia
- Ministry of Water and Energy (MOWE), Addis Ababa, Ethiopia
- National Meteorological Agency (NMA), Addis Ababa, Ethiopia
- Oromiya Agricultural Research Institute (ORARI)
- Overseas Development Institute (ODI), London, United Kingdom
- Research-Inspired Policy and Practice Learning in Ethiopia and the Nile Basin (RiPPLE)
- Stockholm Environment Institute (SEI), Stockholm, Sweden
- University of Bahir Dar, Bahir Dar, Ethiopia
- University of Ambo, Ambo, Ethiopia
- University of Wollega, Wollega, Ethiopia
- World Agroforestry Centre (ICRAF), Addis Ababa, Ethiopia